The kidneys are composed of a (near) million functional units or nephrons working in parallel to perform their homeostatic functions. The complex three-dimensional (3D) architecture of the nephron network, along with the multi-cellular composition as well as distinct functions of nephron segments, challenges our understanding of how the concerted responses of hundreds of thousands of nephrons maintain kidney function, and ultimately whole-body homeostasis. Inter-nephron crosstalk is known to be fundamental in key kidney functions including the regulation of blood flow and glomerular filtration rate(1, 2).