In recent years, the strategy of using laser capture microdissection and mass spectrometry (LCM/MS) has expanded the landscape of antigens associated with membranous nephropathy (MN). Specific associations with phenotypes, diseases, and sometimes reversible triggers led to an antigen-based classification of MN, informing precision medicine and highlighting the potential value of routine use of proteomics in classifying MN. This study reproduces and further improves the original LCM/MS for antigen detection in MN.